Light-induced asymmetries in embryonic retinal gene expression are mediated by the vascular system and extracellular matrix

Autor(en)
Elisabetta Versace, Paola Sgado, Julia George, Jasmine L Loveland, Joseph Ward, Peter Thorpe, Lars J. Jensen, Karen A Spencer, Silvia Paracchini, Giorgio Vallortigara
Abstrakt

Left–right asymmetries in the nervous system (lateralisation) influence a broad range of behaviours, from social responses to navigation and language. The role and pathways of endogenous and environmental mechanisms in the ontogeny of lateralisation remains to be established. The domestic chick is a model of both endogenous and experience-induced lateralisation driven by light exposure. Following the endogenous rightward rotation of the embryo, the asymmetrical position in the egg results in a greater exposure of the right eye to environmental light. To identify the genetic pathways activated by asymmetric light stimulation, and their time course, we exposed embryos to different light regimes: darkness, 6 h of light and 24 h of light. We used RNA-seq to compare gene expression in the right and left retinas and telencephalon. We detected differential gene expression in right vs left retina after 6 h of light exposure. This difference was absent in the darkness condition and had already disappeared by 24 h of light exposure, suggesting that light-induced activation is a self-terminating phenomenon. This transient effect of light exposure was associated with a downregulation of the sensitive-period mediator gene DIO2 (iodothyronine deiodinase 2) in the right retina. No differences between genes expressed in the right vs. left telencephalon were detected. Gene networks associated with lateralisation were connected to vascularisation, cell motility, and the extracellular matrix. Interestingly, we know that the extracellular matrix—including the differentially expressed PDGFRB gene—is involved in morphogenesis, sensitive periods, and in the endogenous chiral mechanism of primary cilia, that drives lateralisation. Our data show a similarity between endogenous and experience-driven lateralisation, identifying functional gene networks that affect lateralisation in a specific time window.

Organisation(en)
Department für Verhaltens- und Kognitionsbiologie
Externe Organisation(en)
Università degli Studi di Trento, University of Copenhagen, Queen Mary University of London, University of St. Andrews, Clemson University
Journal
Scientific Reports (Nature Publisher Group)
Band
12
Anzahl der Seiten
12
ISSN
2045-2322
DOI
https://doi.org/10.1038/s41598-022-14963-8
Publikationsdatum
07-2022
Peer-reviewed
Ja
ÖFOS 2012
106014 Genomik, 106025 Neurobiologie, 106010 Entwicklungsbiologie
Schlagwörter
Link zum Portal
https://ucris.univie.ac.at/portal/de/publications/lightinduced-asymmetries-in-embryonic-retinal-gene-expression-are-mediated-by-the-vascular-system-and-extracellular-matrix(813e6f7c-5e0a-41e8-bbe9-b915f8ff9685).html